Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Biotang Inc HEPES Protein Extraction Buffer (5X), 250 ml
HEPES Protein Extraction Buffer (5X), 250 ml.
Teknova 1M Tris-HCl, pH 7.5 Solution, 20L Bag, Sterile. 4 Pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
![Research Products International Corp TES, Sodium Salt, [2-Tris(hydroxymethyl) methyl-2-amino-1-ethanesulfonic acid, sodium salt], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T31020-100.0.JPG-250.jpg)
Research Products International Corp TES, Sodium Salt, [2-Tris(hydroxymethyl) methyl-2-amino-1-ethanesulfonic acid, sodium salt], 100 Grams
TES (N-[Tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid) Sodium Salt is a commonly used buffering agent in life science research. It helps to maintain a stable pH environment in biological experiments, such as cell culture, protein purification, and enzyme assays.
TES Sodium Salt is advantageous due to its ability to resist changes in pH over a wide range of temperatures, making it suitable for various experimental conditions.
Additionally, its low UV absorbance and minimal interference with biological molecules make it a preferred choice in many biochemical applications
Teknova 20 x MOPS/SDS Running Buffer. 500mL, Sterile. 8 pack.
MOPS (3-(N-morpholino)propanesulfonic acid) is a Good’s buffer and has a pKa of 7.01, making it an ideal buffer for many biological systems at near-neutral pH. MOPS can be used in cell culture media and biopharma buffer formulations as well as in the purification of antibodies, peptides, proteins and blood components. It is nontoxic to cell culture lines.
![Alkali Scientific BES [N,N bis(2 Hydroxyethyl) 2 aminoethanesulfonic acid], 100 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660773.jpg-250.jpg)
Alkali Scientific BES [N,N bis(2 Hydroxyethyl) 2 aminoethanesulfonic acid], 100 G
BES (N,N bis(2 Hydroxyethyl) 2 aminoethanesulfonic acid) is a biochemical reagent commonly used as a buffering agent in laboratory research. The 100g quantity provides a stable and reliable source of BES for a wide range of applications, including protein studies, enzyme assays, and electrophoresis. BES is valued for its ability to maintain a stable pH environment in biochemical systems, especially when working with proteins and other biomolecules sensitive to pH variations. It is commonly used in preparation of buffers for biological assays and for maintaining a consistent pH during biochemical reactions. This reagent is indispensable in labs focused on molecular biology, biochemistry, and clinical diagnostics.
![Alkali Scientific MOPS, Sodium Salt [3 (N Morpholino) propanesulfonic acid sodium salt], 50 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660066.jpg-250.jpg)
Alkali Scientific MOPS, Sodium Salt [3 (N Morpholino) propanesulfonic acid sodium salt], 50 G
MOPS (3-(N-Morpholino)propanesulfonic acid sodium salt) is a versatile biochemical reagent used for pH buffering in various research applications. This 50g bottle provides a compact quantity of MOPS sodium salt, ideal for laboratories with moderate buffer preparation needs. MOPS is widely used in molecular biology, biochemistry, and biotechnology for stabilizing pH in enzyme reactions, protein assays, and cell culture. Its pH buffering capacity at a range of 7.0–7.5 is particularly beneficial in experiments where a consistent environment is essential. MOPS also serves as an essential reagent in electrophoresis, chromatography, and other scientific procedures that demand precise pH control.
Bioworld ADA (N-(2-acetamido)iminodiacetic acid) Buffer, 0.2M, pH 7.0, 100 mL
Synonym:ADA-Na,N-(2-Acetamido)iminodiacetic acid monosodium salt,N-(Carbamoylmethyl)iminodiacetic acid monosodium saltA zwitterionic buffer used in biological applications including protein isolation and characterizationWarning: For laboratory use only.Not fit for agricultural, household, clinical use or human consumption. Appearance: LiquidPH Value: 7.0
Medchemexpress LLC L-Adenosine | 3080-29-3 | 99.8% | 1 ML
L-Adenosine is a metabolically stable enantiomeric analog and a potential probe. It exhibits weakly inhibitory adenosine deaminase (ADA) activity with a Ki value of 385 μM. This compound can be utilized for research into adenosine uptake and accumulation.
- Metabolically stable enantiomeric analog.
- Potential probe for research.
- Weakly inhibits adenosine deaminase activity.
- Can be used for adenosine uptake research.
- Can be used for adenosine accumulation research.