Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Teknova 6X SSC Molecular Biology Grade Solution. 500mL, Sterile. 4 pack.
6X SSC Molecular Biology Grade Solution. 500mL, Sterile. 4 pack.
Boston Bioproducts Inc Molecular Biology Grade Water - 2x1L
Molecular Biology Grade Water - 2x1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (pH 7.4) - 2x1L
Bioprocess Grade - Phosphate Buffered Saline PBS (pH 7 4) - 2x1L (For Research Use and Further Manufacturing Only)
Teknova 0.45% Sterile Saline, 6mL, 25-Pack, Sterile. 12 Pack.
Sodium chloride is an essential ingredient in the lab. Not only does it help regulate the pH of solutions, but it also prevents growth of bacteria and other contaminants. In addition, salt is a powerful desiccant, meaning that it can help to dry out wet samples. As a result, salt is often used in a wide variety of laboratory procedures.
Alkali Scientific Saline 0.85% 3mL 13x100mm Polycarbonate Screw Cap Tube, 1/Pk
Saline 0.85% 3mL 13x100mm Polycarbonate Screw Cap Tube, 1/Pk is a sterile isotonic saline solution designed for microbiological and clinical laboratory applications. The 0.85% sodium chloride concentration provides osmotic balance that maintains cell integrity without supplying nutrients that could promote microbial growth. This makes the solution suitable for preparing microbial suspensions, performing serial dilutions, rinsing cells, and supporting sample handling procedures. Each tube is prefilled with 3mL of sterile saline and packaged in a durable polycarbonate screw cap tube that helps prevent contamination while ensuring convenient handling. The single use format supports reliable, consistent workflows in clinical diagnostics, microbiology laboratories, and research environments.
Discovery Life Sciences .5M POTASSSIUM PHOS BUFFER 7.4
Potassium Phosphate Buffer, P450 Enzyme Buffer, 0.5M
Boston Bioproducts Inc Paraformaldehyde (4%, PBS) - 250ml
Paraformaldehyde solution is a neutral fixative solution used to fix tissues and cells in immunohistochemistry and immunocytochemistry protocols. Stabilized in a phosphate buffer for delivery, this reagent actively cross-links proteins and DNA, immobilizing cellular components and maintaining the integrity of cell morphology. This reagent supports the accurate visualization of cells and tissues under a microscope, ensuring that researchers can observe fine details without degradation.
Bioworld Carbonate-buffered saline pH 9.5 (5X), 500 mL
This buffer contains 125mM sodium carbonate and 750mM sodium chloride, pH 9.5. The buffer is important for several different cellular processes. It is one of the major buffering systems used to maintain the pH of mammalian blood and is also important in the acid-base homeostasis of living things, including humans. It tends to maintain a relatively constant plasma pH and counteract any force that would alter it.Warning:For laboratory use only.Not fit for agricultural, household, clinical use or humanconsumption. Appearance: LiquidPH Value: 9.5
Medchemexpress LLC PBS Buffer (10x) | 1 L
Phosphate-Buffered Saline (PBS) is a balanced salt solution primarily utilized for various cell culture applications, including washing cells, diluting cells, and preparing reagents. MedChemExpress's PBS Buffer (10x) is filtered, free of Mg2+, Ca2+, and phenol red, and has a pH ranging from 7.2 to 7.4.
- Filtered
- Free of Mg2+
- Free of Ca2+
- Free of phenol red
- pH is 7.2-7.4
Seracare Life Sciences Inc 10X PHOSPHATE BUFFERED SALINE
10X Phosphate-buffered Saline (PBS) is ideal for use in sample preparation and as a wash buffer in general immunoassay applications. pH=7.4. Conductivity controlled.
Apexbio Technology LLC Annexin V-PE Apoptosis Kit 20tests
Annexin V-PE Apoptosis Kit enables differentiation between early apoptotic late apoptotic and dead cells Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) PS is normally located on the inner leaflet of the cell membrane phospholipid bilayer However during the early stages of apoptosis PS moves from the inner surface to the outer surface of the cell membrane becoming exposed to the extracellular environment Labeled with a fluorescence dye such as PE Annexcin V is commonly used as a fluorescent probe to detect apoptosis through flow cytometry or fluorescence microscopy within 15-30 minutes Alternatively you can directly use our ready-to-use dual-staining kits Annexin V-PE/7-AAD Apoptosis Kit ( K2283) or Annexin V-PE/DAPI Apoptosis Kit (K2282)
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (10X, for Western Blot Washing) - 1L
Phosphate Buffered Saline PBS (10X for Western Blot Washing) - 1L (For Research Use and Further Manufacturing Only)